Composite

Part:BBa_K1740004:Design

Designed by: Keisuke Sugahara   Group: iGEM15_KAIT_Japan   (2015-09-06)


pLac+RBS+145N+MCS+145N+stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 959
    Illegal XhoI site found at 899
    Illegal XhoI site found at 911
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

none

References

[1] Xin X. Zhou, Hokyung K. Chung, Amy J. Lam, and Michael Z. Lin ;Optical control of protein activity by fluorescent protein domains

[2] Jared E. Toettcher, Delquin Gong, Wendell A. Lim, and Orion D. Weiner ;Light control of plasma membrane recruitment using the Phy-PIF system

[3] Hideaki Mizuno, Tapas Kumar Mal, Markus Walchli, Akihiro Kikuchi, Takashi Fukano, Ryoko Ando, Jeyaraman Jeyakanthan, Junichiro Taka, Yoshitsugu Shiro, Mitsuhiko Ikura, and Atsushi Miyawaki ;Light-dependent regulation of structural flexibility in a photochromic fluorescent protein

[4] Martin Andresen, Andre C Stiel, Jonas Folling, Dirk Wenzel, Andreas Schonle, Alexander Egner, Christian eggeling, Stefan W Hell and Stefan Jakobs; Photoswitchable fluorescent proteins enable monochoromatic multilabel imaging and dual color fluorescence nanoscopy

[5] Xin x. Zhou and Michael Z. Lin; Photoswitchable Fluorescent Proteins: TenYears of Colorful Chemistry and Exciting Applications

[6] E. N. Baker and Z. Dauter; Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa